Molecular diagnostics is the study and analysis of human genes to better understand the nature of diseases, like who is most susceptible to them and the soundest ways to treat or control them. In addition, there are gene mutations associated with certain diseases. This compelling field of genetics leads us towards more personalized treatment with a visionary approach.
Supplies And Equipment Needed In Molecular Diagnostic Labs
Specific supplies and tools are required in molecular diagnostic labs to examine possible red flags in genetic code sequencing. Some techniques include PCR (Polymerase Chain Reaction) amplification, sequencing, STR analysis, and nucleic acid isolation and quantification. Molecular diagnostic labs generally include these supplies and equipment:
- Refrigerators– Refrigerators are required to hold chemicals and PCR products at average temperatures of around 4 degrees Celsius. PCR is employed to analyze gene expression, clones, sequences, mutagenesis, and genotyping.
- Freezers– Freezers are utilized to hold frozen tissue cultures and samples. You can use a refrigerator/freezer combo unit or purchase them separately depending on available space and the area required for sample storage. The temperature range needs to be between -20 degrees Celsius and -80 degrees Celsius.
- Vortex Mixers– Vortex mixers are operated to mix liquid elements in tubes (like mixing small vials) and the resuspension of cells. These mixers are typically developed with touch or continuous modes and electronic speed management for consistent speeds.
- Centrifuges– Molecular diagnostic labs should include refrigerated centrifuges with 5000 rpms and non-refrigerated centrifuges, depending on the temperature required—Centrifuges separate components based on their density. Microcentrifuges are also operated and should include at least 15,000 rpms for 1.5 ml tubes.
- Thermal Cyclers– Thermal cyclers are required to study the PCR. They include heating, cooling, and accurate temperature controls that guarantee temperature consistency to amplify components of DNA via PCR.
- Spectrophotometers– Spectrophotometers determine nucleic acid quantification by gauging light absorption or the number of chemicals in solutions. A light beam passes through the specimen, and the compounds transfer light over a wavelength.
- DNA Sequencers– Sequencers are employed to observe DNA’s molecular sequence. The resulting patterns of DNA bands or sequences demonstrate how enzymes affect DNA. DNA sequencers determine the order of cytosine, guanine, adenine, and thymine, also known as a text string.
- Microscopes– Microscopes observe and evaluate samples and are usually equipped with camera systems for archiving test results.
- Electrophoresis Systems– An electrophoresis system is utilized in PCR analysis. Molecular motion and separation are observed during the electrophoresis operation.
- Autoclaves– Autoclaves are employed in molecular diagnostic labs for sterilizing waste and lab supplies. Sterilization is performed using heat to eradicate bacteria and spores.
- Incubators– Incubators maintain optimal conditions like temperature and humidity when developing and maintaining cultures by creating an ideal environment.
Molecular diagnostic labs have a significant impact on patient treatment. As a result, communicable diseases are treated sooner and more accurately. These labs use techniques to investigate biological markers in the proteome and genome, including how cells express their genes as proteins. Finally, diseases are identified by analyzing DNA, RNA, and proteins in a fluid or a tissue. Molecules, genes, and cellular operations become irregular and reveal specific diseases.
These are just some of the lab supplies and equipment required for molecular diagnostics. Contact us today to learn more about our medical laboratory supplies. We are here for you!